Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro

doi: 10.1155/2018/2025914

Figure Lengend Snippet: The primary antibodies used in this study.

Article Snippet: CDK5 , ab40773 , AB_726779 , Rabbit , 1 : 3000 , Abcam.

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro

doi: 10.1155/2018/2025914

Figure Lengend Snippet: DAU ameliorated tau pathology via PP2A and p35/25 in both N2a/APP cells and HEK293/Tau cells. Phosphorylation of GSK3 and PP2A and levels of p35/25 and CDK5 in N2a/APP cells (a, b) and HEK293/Tau cells (c, d) as determined by Western blot analysis. β -Actin was used as a loading control. N = 3. Data show the mean ± SEM. ∗∗ P < 0.01 compared to N2a/WT cells, # P < 0.05 and ### P < 0.001 compared to untreated N2a/APP cells, and && P < 0.01 and &&& P < 0.001 compared to untreated HEK293/Tau cells.

Article Snippet: CDK5 , ab40773 , AB_726779 , Rabbit , 1 : 3000 , Abcam.

Techniques: Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro

doi: 10.1155/2018/2025914

Figure Lengend Snippet: The mode of action of DAU. DAU treatment suppressed AD-related changes, notably A β accumulation and tau phosphorylation via APP processing and the CDK5, PP2A, and p35/25 pathways, which may be attributed to the modification of proteins related to functions of oxidative stress, ER stress, molecular chaperones, and signaling protein.

Article Snippet: CDK5 , ab40773 , AB_726779 , Rabbit , 1 : 3000 , Abcam.

Techniques: Modification